Non-invasive deep brain stimulation therapies for neuroregeneration

Abstract Neurodegenerative diseases, such as Alzheimer’s disease, constitute a major socioeconomic burden. As clinical prevalence dramatically increases, the need for disease-modifying therapies is pressing. In this context, regenerative medicine are considered promising strategy treatment of these patients, due to low turnover neural tissues and extensive neurodegeneration. The transplant exogenous stem cells has been demonstrated improve symptoms lead functional recovery.However, high immunogenicity cell survival limit efficacy (Duncan et al.,2017). Electrical stimulation shown modulate proliferation differentiation (NSC), thus, it holds therapeutic potential (Thrivikraman al.,2018). Although therapies, deep brain (DBS) have widely used in clinic, invasiveness approach precludes translation.Moreover, mechanisms underlying remain unknown (Lozano al.,2019). DBS via temporally interfering (TI) electric fields emerged non-invasive (Grossman However, its effect on neuroregeneration biology remains unexplored. Here, influence TI primary NSCs was investigated vitro. Transcriptome analysis using mRNA-seq carried out investigate changes gene expression following stimulation. It that induced genes involved development cellular metabolism. addition, immunostaining showed led upregulation biomarkers associated with differentiation. Taken together, our results suggest could be direct NSC fate through modulation metabolic pathways proliferation. This mechanism action partly explain benefits basis targeted strategies neurodegenerative disorders. Research Category Technology Methods Translational Research: 25. Therapeutics Keywords: Neuroregeneration, Deep Brain Stimulation, Non-invasive stimulation, Stimulation